2021-07-08 18:45:36
Important Points of the Chapter "Biotechnology: Principles and Process"
The first recombination DNA made by Stanley Cohen and Herbert Boyer(1972) by linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA ) of Salmonella typhimurium into E.coli
Restriction enzymes belong class of enzymes called nucleases.
There are two types of restriction enzymes: Endonuclease (cuts at specific positions within the DNA ) & Exonucleases (remove nucleotides from the ends of the DNA)
Palindromic nucleotide sequences: The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.
Gel Electrophoresis: It is a technique to separate out DNA fragments formed by restriction endonuclease
Plasmids: it is a circular extra-chromosomal DNA of bacteria, having antibiotic resistance gene.
Micro-injection: It is a technique of injecting recombinant DNA is directly into the nucleus of an animal cell.
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